Effect of small interfering RNAs on matrix metalloproteinase 1 expression

Effect of small interfering RNAs on matrix metalloproteinase 1 expression

Three small double strand siRNAs (506-MMP1, 859-MMP1 and 891-MMP1), each contains 25-26 nucleotides, with high specific to human MMP1 were designed according to mRNA sequence of human MMP1 (NCBI, NM_002421). To monitor the MMP1 gene expression, the total RNAs of human skin fibroblast (Detroit 551, BCRC 60118) were extracted. One human matrix metalloproteinase 1 (MMP1) partial sequence cDNA, inc...

Effect of small interfering RNAs on in vitro replication and gene expression of feline coronavirus.

OBJECTIVE To evaluate the ability of small interfering RNAs (siRNAs) to inhibit in vitro viral replication and gene expression of feline coronavirus (FCoV). SAMPLE Cell cultures of Crandell-Rees feline kidney cells. PROCEDURES 5 synthetic siRNAs that each targeted a different region of the FCoV genome were tested individually and in various combinations for their antiviral effects against 2...

Effect of Foretinib on Matrix Metalloproteinase-2 (MMP2) Expression in Glioblastoma

Background: The most malignant form of infiltrating astrocytoma, glioblastoma multiforme (GBM), is one of the most aggressive human cancers. Foretinib diminished GBM cell invasion by downregulating the expression of matrix metalloproteinase 2 (MMP2). The study aimed to examine the anti-tumor activity of foretinib and to test its effect on MMP2 expression in T98 cells. Materials and methods: T9...

stably silencing of cd81 expression by small interfering rnas targeting 3'-ntr inhibits hcv infection

Expression of Matrix Metalloproteinase-2 and -9 in Meningioma

Background: Meningioma is one of the most common tumors of the central nervous system. It was shown that meningioma had up-regulated expression of Matrix Metalloproteinases (MMPs) that involved in cell growth, angiogenesis and metastasis.  Objectives: The aim of the study was the assessment of serum MMP-2 and -9 levels in patients with different grades of meningioma.  Materials & Me...